Human RalA/RalB antibody

Item no.	NKM-ATGA0590-50ul
Manufacturer	NKMAX
Amount	50 ul
Quantity options	100 ul 50 ul
Category	Antibodies Cell Biology Cytoskeleton Flow Cytometry Western Blot ELISA Primary Antibodies Immunostaining small GTPases Ras Subfamily
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, ELISA
Clone	AT41E2
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b Kappa
Conjugate/Tag	Unconjugated
Citations	Balasubramanian N., et al. (2009) Curr. Biol. 20:75-79. Hunt MC., et al. (2005) J Lipid Res. 46, 2029-32. Wildey G.M., et al. (1993), Biochem. Biophys. Res. Comm., 194: 552-559. Jullien-Flores V., et al. (1995). J. Biol. Chem. 270: 22473-22477.
NCBI	NP_005393
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Ras-related protein Ral-A,RAL,Ras-related protein Ral-B
Available
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, FACS
Manufacturer - Category	Monoclonal antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	RalA and RalB belong to subfamily of Ras-related GTPases. This protein mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. It is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicletrafficking, and endocytosis. RalA and RalB share 85% identity.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human RALA (1-203aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Epigenetics and Nuclear Signaling
Manufacturer - Search Terms	RalB, Ras-related protein Ral-B, Ras related protein RalB, Ras related protein Ral B, RalA, Ras-related protein Ral-A, Ras related protein RalA, Ras related protein Ral A, ATGA0590-10ug, ATGA0590-20ug, ATGA0590-50ug, ATGA0590-100ug, ATGA0590-250ug, ATGA0590-500ug, ATGA0590-1mg, ATGA0590-010, ATGA0590-020, ATGA0590-050, ATGA0590-100, ATGA0590-250, ATGA0590-500, ATGA0590-01M
Manufacturer - Similar Names	RalB, Ras-related protein Ral-B, Ras related protein RalB, Ras related protein Ral B, RalA, Ras-related protein Ral-A, Ras related protein RalA, Ras related protein Ral A, ATGA0590-10ug, ATGA0590-20ug, ATGA0590-50ug, ATGA0590-100ug, ATGA0590-250ug, ATGA0590-500ug, ATGA0590-1mg, ATGA0590-010, ATGA0590-020, ATGA0590-050, ATGA0590-100, ATGA0590-250, ATGA0590-500, ATGA0590-01M
WB Comment	The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human RalA protein Lane 2.: Recombinant human RalB protein The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: NIH3T3 cell lysate Lane 4.: PC3 cell lysate Lane 5.: U87MG cell lysate
FACS Comment	Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained with ATGA0590 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

NKM-ATGA0590-50ul-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 50 ul

available

Delivery expected until 12/4/2025

Express Delivery: Save 9 days

Compare

Add to wishlist

Get an offer