Comparison

Rabbit antibody to ATG7

Item no. OSA00019W
Manufacturer Osenses
Amount 150ul
Category
Type Antibody Polyclonal
Applications WB, IHC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias APG7-like, APG7L, hAGP7, APG7L, ubiquitin-activating enzyme E1-like protein
Similar products APG7L, hAGP7, APG7-like, ubiquitin-activating enzyme E1-like protein
Available
Also known
APG7-like, APG7L, hAGP7, APG7L, ubiquitin-activating enzyme E1-like protein
Target
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy.
SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
Spcs X-react.
Human, mouse and rat. Other species not yet tested.
Applications info
IHC, WB. Use at a dilution of 1 : 1000. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Tanida I, et al. FEBS J. 273 (11), 2553-2562(2006).
Limitation
For research use only
Immunogen
A synthetic peptide from c-terminal region of human ATG7 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in rat and mouse.
Conjugate
Unconjugated antibody
Accession
http://ca.expasy.org/uniprot/O95352
http://ca.expasy.org/uniprot/Q641Y5
http://ca.expasy.org/uniprot/Q9D906
Purity
Whole serum
Clonality
Polyclonal
Specificity
Specific for ATG7.
Format
Lyophilised
Reconstitution
Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 150ul
Available: In stock
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