Comparison

Rabbit antibody to AKT

Item no. OSA00058W
Manufacturer Osenses
Amount 100ul
Category
Type Antibody Polyclonal
Applications WB, IHC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, PKB, RAC, AKT1
Similar products AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, Protein kinase B, RAC-PK-alpha, C-AKT
Available
Also known
RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, PKB, RAC, AKT1
Target
FUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.
TISSUE SPECIFICITY: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.
Spcs X-react.
Mouse, rat, human. Other species not yet tested.
Applications info
IHC, WB. A dilution of 1 : 1000 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. This antibody is also very likely to recognise the unphosphorylated form.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Cong L.N, et al. Mol. Endocrinol. 11:1881-1890(1997).
Limitation
For research use only
Immunogen
A synthetic peptide from mouse AKT conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, human and chicken.
Conjugate
Unconjugated antibody
Accession
http://ca.expasy.org/uniprot/P31750
http://ca.expasy.org/uniprot/P47196
http://ca.expasy.org/uniprot/P31749
http://ca.expasy.org/uniprot/O57513
Purity
Whole serum
Clonality
Polyclonal
Specificity
Specific for AKT1, AKT2, AKT3.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100ul
Available: In stock
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