Rabbit antibody to MBP

FUNCTION: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic side of myelin.
TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

IHC, WB. A dilution of 1: 1000 is recommended for IHC-P and 1:2000 for WB. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Roth H.J, et al. J. Neurosci. Res. 16:227-238(1986)
2. Li Y, et al. EMBO J. 24:2968-2979(2005)

A synthetic peptide from human MBP conjugated to blue carrier protein was used as the antigen.

http://www.uniprot.org/uniprot/P02686
http://www.uniprot.org/uniprot/P02688
http://www.uniprot.org/uniprot/P04370

Polyclonal

Lyophilised