Rabbit antibody to Rhodopsin

FUNCTION: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
BIOPHYSICOCHEMICAL PROPERTIES:
Absorption: Abs(max)=495 nm;
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Rod shaped photoreceptor cells which mediates vision in dim light.
PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
DISEASE: Defects in RHO are the cause of retinitis pigmentosa type 4 (RP4). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP4 inheritance is autosomal dominant.
DISEASE: Defects in RHO are a cause of retinitis pigmentosa autosomal recessive (ARRP).
DISEASE: Defects in RHO are a cause of autosomal dominant congenital stationary night blindness 1 (CSNBAD1); also known as rhodopsin-related congenital stationary night blindness. Congenital stationary night blindness is a nonprogressive retinal disorder characterized by impaired night vision and ocular symptoms such as myopia, hyperopia, nystagmus and reduced visual acuity.

IHC, WB. A dilution of 1:2000 is recommended for IHC-P and 1:5000 to 1:7000 for WB. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Nathans J, et al. Proc. Natl. Acad. Sci. U.S.A. 81:4851-4855(1984).

A synthetic peptide from human Rhodopsin conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and rat.

http://www.uniprot.org/uniprot/P08100
http://www.uniprot.org/uniprot/P15409
http://www.uniprot.org/uniprot/P51489

Polyclonal

Lyophilised