Rabbit antibody to Septin 2

FUNCTION: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm a€C cytoskeleton. Cytoplasm a€C cytoskeleton a€C spindle. Note: In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. In epithelial cells, colocalizes with polyglutamylated tubulin around the trans-Golgi network, as well as juxatnuclear and proximal Golgi apparatus.
TISSUE SPECIFICITY: Widely expressed. Up-regulated in liver cancer.

IHC, WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Nagase T, et al. DNA Res. 2:167-174(1995)

A synthetic peptide from the c-terminal region of human Septin 2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

http://www.uniprot.org/uniprot/Q15019
http://www.uniprot.org/uniprot/Q91Y81
http://www.uniprot.org/uniprot/P42208

Polyclonal

Lyophilised