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SARS-CoV-2 Spike NTD Antibody [2146]

SARS-CoV-2 Spike NTD Antibody [2146]

Item no.	PRS-10-055-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Infectious Diseases Viral Infections Coronavirus (COVID-19) Antibodies Immunohistochemistry ELISA Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	IHC, ELISA
Clone	2146
Specific against	other
Isotype	IgG1
Conjugate/Tag	Unconjugated
Citations	Ou, X., Liu, Y., Lei, X. et al. Nat Commun 11, 1620. 2020. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Coronaviridae family 1. The SARS-CoV-2 genome, which shares 79.6% identity with SARS-CoV, encodes four essential structural proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) 2. The S protein is a transmembrane, homotrimeric, class I fusion glycoprotein that mediates viral attachment, fusion, and entry into host cells 3. Each ~180 kDa monomer contains two functional subunits, S1 (~700 a.a) and S2 (~600 a.a), that mediate viral attachment and membrane fusion, respectively. S1 contains two major domains, the N-terminal (NTD) and C-terminal domains (CTD). In both SARS-CoV and SARS-CoV-2, the CTD contains the receptor-binding domain (RBD), which binds to the angiotensin-converting enzyme 2 (ACE2) receptor on host cells3-5. The NTD of SARS-CoV-2 does not bind to ACE26, and the function of NTD in SARS-CoV-2 infection is not well understood. In other CoVs, the NTD may promote attachment by binding to sugar moieties7 and might play a role in the conformational change of S2 required for membrane fusion8. While most neutralizing antibodies target the RBD domain and block receptor binding, potent neutralizing antibodies targeting NTD were isolated from convalescent COVID19 patients9, identifying the NTD as an attractive candidate for vaccines and therapeutics. In addition, the NTD is a promising antigen for diagnostic tests, as there is only 53.5% homology between the NTD of SARS-CoV-2 and SARS-CoV10. Zhou, P., Yang, X., Wang, X. et al. Nature 579, 270–273. 2020. Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265–269. 2020. Wrapp D, Wang N, Corbett KS, et al. bioRxiv. 2020. Walls AC, Park YJ, Tortorici MA, et al. Cell. 181(2):281-292.e6. 2020. Li W, Zhang C, Sui J, et al. EMBO J. 24(8):1634-1643. 2020. Wang Q, Zhang Y, Wu L, et al. Cell. 181(4):894-904.e9. 2020. Park, Y., Walls, A.C., Wang, Z. et al. Nat Struct Mol Biol 26, 1151–1157. 2019. Zhou, H., Chen, Y., Zhang, S. et al. Nat Commun 10, 3068. 2019. Chi X, Yan R, Zhang J, et al. Science. eabc6952. 2020.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	SARS-CoV2 Spike NTD, COVID-19, 2019-nCoV, Severe acute respiratory syndrome coronavirus 2, SARS-CoV2
Available
Manufacturer - Applications	ELISA (Quality Tested by ProSci); IHC (Other applications as reported in literature)
Manufacturer - Isotype	Human IgG1
Shipping Temperature	blue ice or room temperature
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8° C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80° C. Avoid Repeated Freeze Thaw Cycles.
Manufacturer - Research Area	Infectious Disease, COVID-19
Protein Gi #	SARS-CoV-2
Purification	>95% by SDS-PAGE and HPLC
Concentration	>1.0 mg/ml
Buffer	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Immunogen	Sequenced from human survivors of COVID-19 (SARS-CoV-2). The spike NTD is expressed on the surface of SARS-CoV-2.
Clonality	Monoclonal
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 9/4/2025

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