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LEF1 Antibody

LEF1 Antibody

Item no.	PRS-27-587-100ul
Manufacturer	ProSci
Amount	100 ul
Category	Antibodies Immunohistochemistry Western Blot ELISA Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IHC, ELISA
Specific against	Human (Homo sapiens), Dog (Canine, Canis lupus familiaris)
Host	Rabbit
Conjugate/Tag	Unconjugated
Citations	Liu, X., et al., (2004) J.Invest.Dermatol.123(2), 264-274.
NCBI	LEF1
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	LEF1, LEF-1, TCF10, TCF7L3, TCF1ALPHA
Available
Manufacturer - Applications	LEF1 antibody can be used for detection of LEF1 by ELISA at 1:62500. LEF1 antibody can be used for detection of LEF1 by western blot at 0.1-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Shipping Temperature	blue ice or room temperature
Storage Conditions	For short periods of storage (days) store at 4°C. For longer periods of storage, store LEF1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Molecular Weight	44 kDa
Manufacturer - Research Area	Transcription , Cancer , Signal Transduction
Background	Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1).
Protein Gi #	7705917
Purification	Antibody is purified by peptide affinity chromatography method.
Concentration	Batch dependent
Buffer	Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Immunogen	Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1.
Clonality	Polyclonal
NCBI Official Name	lymphoid enhancer-binding factor 1
NCBI Organism	Homo sapiens
Disclaimer	This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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