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ATG5 Antibody

ATG5 Antibody

Item no.	PRS-30-955-100ul
Manufacturer	ProSci
Amount	100 ul
Category	Antibodies Cell Biology Cell Status Western Blot ELISA Autophagy Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rabbit
Conjugate/Tag	Unconjugated
Citations	Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).
NCBI	ATG5
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ATG5,APG5,APG5L,ASP,hAPG5,APG5-LIKE
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATG5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Molecular Weight	32 kDa
Manufacturer - Research Area	Cancer , Apoptosis
Background	ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
By Type	Cancer, Apoptosis
Modifications	None
Protein Gi #	4757798
Purification	Antibody is purified by peptide affinity chromatography method.
Positive Control 1	Cat. No. 1211 - HepG2 Cell Lysate
Concentration	batch dependent
Buffer	Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Application Note	ATG5 antibody can be used for detection of ATG5 by ELISA at 1:1562500. ATG5 antibody can be used for detection of ATG5 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Immunogen	Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATG5.
Clonality	Polyclonal
NCBI Official Name	autophagy related 5
NCBI Organism	Homo sapiens
Disclaimer	This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 1/1/2026

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