Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Canine |
Format |
Liquid |
Applications |
WB, ELISA |
Amount |
100 ul |
Host |
Rabbit |
Item no. |
PRS-31-189-100ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Transcription , Signal Transduction |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2F1. |
Applications |
GTF2F1 antibody can be used for detection of GTF2F1 by ELISA at 1:312500. GTF2F1 antibody can be used for detection of GTF2F1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1204 - K562 Cell Lysate |
Predicted Molecular Weight |
58 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GTF2F1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
GTF2F1 |
Accession # |
NP_002087 |
Protein Gi # |
156104891 |
Ncbi Gene Id # |
2962 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
GTF2F1 |
Ncbi Official Full Name |
general transcription factor IIF, polypeptide 1, 74kDa |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P35269 |
Background |
GTF2F1 is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. |
Background References 1 |
Zhang, C., et al., (2005) Mol. Cell. Biol. 25 (9), 3583-3595. |
1st Image Caption |
Antibody used in WB on Human K562 at 0.2-1 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.