Comparison

ApoM Antibody [8F12C6B8]

Item no. PRS-32-112-0.1mL
Manufacturer ProSci
Amount 0.1 mL
Category
Type Antibody Primary
Applications WB, ELISA
Clone 8F12C6B8
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1, IgG2b
Conjugate/Tag Unconjugated
Citations Xu, N. & Dahlback, B. 1999 J. Biol. Chem. 274:31286.
Duan J, Dahlback B, Villoutreix BO.FEBS Lett. 2001 Jun 15;499(1-2):127-32.
Xu,N., Nilsson-Ehle,P. & Ahren,B. 2004. J. Nutr.Biochem. 15 (10):579-582.
Zhang,X.Y. ,et al.2004. Acta Histochem. 106 (2):123-128
NCBI APOM
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias G3A,NG20,HSPC336,Protein G3a,Apo-M,G3a,apo-M
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Manufacturer - Isotype
IgG1 & IgG2b
Shipping Temperature
Blue Ice
Storage Conditions
ApoM monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Background
ApoM(apolipoprotein M, also designated G3a or NG20), with 188-amino acid protein(about 21 kDa), is an apolipoprotein and member of the lipocalin protein family.The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro, Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs, and also found in triglyceride-rich lipoproteins and LDLs.
By Type
Other
Modifications
None
Protein Gi #
17370872
Purification
Antibody is purified by protein A affinity chromatography.
Concentration
batch dependent
Buffer
Purified antibody in PBS containing 0.03% sodium azide.
Application Note
Western Blot:1:500 - 1:2, 000
IF:1:200 - 1:1, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Immunogen
Ni-NTA purified full-length recombinant ApoM.trx expressed in E. Coli strain BL21 (DE3).
Clonality
Monoclonal
NCBI Official Name
apolipoprotein M
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mL
Available: In stock
available

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