Comparison

CD14 Antibody [5A3H5D5(FC) / 5A3B11B5]

Item no. PRS-32-128-0.1mL
Manufacturer ProSci
Amount 0.1 mL
Category
Type Antibody Primary
Applications IHC, ELISA
Clone 5A3H5D5(FC) / 5A3B11B5
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
Conjugate/Tag Unconjugated
Citations J Mammary Gland Biol Neoplasia 2000, 5 : 227-241.
J Mammary Gland Biol Neoplasia 2000, 5 : 165-185.
NCBI CD14
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Myeloid cell-specific leucine-rich glycoprotein
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
CD14 monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Manufacturer - Research Area
Innate Immunity
Background
CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.
By Type
Innate Immunity
Modifications
None
Protein Gi #
20141203
Purification
Antibody is purified by protein G affinity chromatography and resuspended in PBS with 0.2%BSA,
0.09% sodium azide. No reconstitution is necessar Concentration is 0.4mg/ml.
Concentration
batch dependent
Buffer
Purified antibody in PBS containing 0.03% sodium azide.
Application Note
IHC:Dilution 1:200 - 1:400
ELISA:Determining optimal working dilutions by titration test.
Immunogen
Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).
Clonality
Monoclonal
NCBI Official Name
CD14 molecule
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mL
Available: In stock
available

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