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Cytokeratin Antibody [7H8C4]

Cytokeratin Antibody [7H8C4]

Item no.	PRS-32-143-0.1mL
Manufacturer	ProSci
Amount	0.1 mL
Category	Antibodies Cell Biology Cytoskeleton Immunohistochemistry Western Blot ELISA Primary Antibodies Intermediate filaments
Type	Antibody Primary
Applications	WB, IHC, ELISA
Clone	7H8C4
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
Citations	Scope A. Schwendenwein I. Frommlet F. Vet Rec. 2006, Dec 16, 159(25): 839-43. Somjen D. Katzburg S. Posner GH. et al. J Cell Biochem. 2007, Apr 15, 100(6): 1406-14.
NCBI	KRT5
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	58 kDa cytokeratin,CK-5,K5,CK5,DDD,DDD1,EBS2,KRT5A
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Cytokeratin(Pan) monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Background	Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.
By Type	Other
Modifications	None
Protein Gi #	143811411
Concentration	batch dependent
Buffer	Ascitic fluid containing 0.03% sodium azide.
Application Note	Western Blot:1:500 - 1:2, 000 IHC(P):1:500 - 1:2, 000 ELISA:Propose dilution 1:10, 000. Determining optimal working dilutions by titration test.
Immunogen	Ni-NTA purified truncated recombinant CK expressed in E. Coli strain BL21 (DE3).
Clonality	Monoclonal
NCBI Official Name	keratin 5
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mL

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Delivery expected until 11/6/2025

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