Caspase-3 (Pro and Active) Antibody [CAS3836]

Primary Antibodies

Aliquot and Store at -20˚ C. Avoid freez-thaw cycles.

Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12). Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins.

None

0.5 mg/mL

Western blot: 2-4 ug/ml
This antibody will detect the pro form and p17 and p12 active forms of Caspase-3.

Monoclonal

homo sapiens

Optimal dilutions for each application to be determined by the researcher