Comparison

GAPDH Antibody [GAP259]

Item no. PRS-33-017-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications WB
Clone GAP259
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Dog (Canine, Canis lupus familiaris), Primate
Host Mouse
Isotype IgG1 Kappa
Conjugate/Tag Unconjugated
NCBI GAPDH
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase,G3PDH,GAPD
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at -20˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Signal Transduction, Neuroscience
Background
Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
By Type
Signal Transduction, Neuroscience
Modifications
None
Purification
Protein G Affinity Chromatography
Concentration
0.5 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Western blot: 0.5-1 ug/ml
Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.
Immunogen
A recombinant protein fragment from C-terminal GAPDH was used as the immunogen.
Clonality
Monoclonal
NCBI Official Name
glyceraldehyde-3-phosphate dehydrogenase
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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