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Alpha Fetoprotein Antibody [C3]

Alpha Fetoprotein Antibody [C3]

Item no.	PRS-33-066-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Targets for Rodent Neurosciences Antibodies By Cell Type Carcinoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies By Organ Lung Cancer Hepatic Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, FC, IF, IHC
Clone	C3
Specific against	Human (Homo sapiens), Monkey (Cynomolgus, Simian), Dog (Canine, Canis lupus familiaris), Pig (Porcine, Sus scrofa domesticus)
Host	Mouse
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
NCBI	AFP
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	AFP Antibody: FETA,HPAFP,Alpha-fetoprotein,Alpha-1-fetoprotein
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Homeostasis
Background	This antibody recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
By Type	Homeostasis
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml IHC(FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance. 1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Manufacturer - Specificity	Does not react with cow, cat, mouse, rat.
Immunogen	Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.
Clonality	Monoclonal
NCBI Official Name	alpha-fetoprotein
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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