CD47 Antibody [B6H12.2]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

None

0.2 mg/mL

Functional Activity (Blocking): Order BSA/Azide-free format
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Western blot: 2-5 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher