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CD50 Antibody [186-2G9]

CD50 Antibody [186-2G9]

Item no.	PRS-33-143-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Protein Aggregation, Clearance & Misfolding Neural Development Antibodies By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Adenocarcinoma Flow Cytometry Immunohistochemistry Immunofluorescence Blocking Primary Antibodies By Organ Bladder Cancer Colorectal Cancer Heart & Blood Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC, Blocking
Clone	186-2G9
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b Kappa
Conjugate/Tag	Unconjugated
NCBI	ICAM3
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Intercellular adhesion molecule 3,ICAM-3,CDw50,ICAM-R,CD50,ICAM3
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Immunology, Signal Transduction
Background	This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI). It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.
By Type	Immunology, Signal Transduction
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Functional Activity (Blocking): Blocks CD50-CD11a interaction Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD50 antibody to be titered up or down for optimal performance. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen	Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
Clonality	Monoclonal
NCBI Official Name	intercellular adhesion molecule 3
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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