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EpCAM Antibody [323/A3]

EpCAM Antibody [323/A3]

Item no.	PRS-33-173-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Neural Lineage Markers Antibodies By Cell Type Carcinoma Cell Biology Cytoskeleton Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Intermediate filaments
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC
Clone	323/A3
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	Unconjugated
NCBI	EPCAM
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Available
Manufacturer - Applications	Flow Cytometry: 0.5-1 ug/million cells IF: 1-2 ug/ml IHC (FFPE): 0.5-1.0 ug/ml for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance. 1. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer, Cell Cycle, Signal Transduction
Background	Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Immunogen	MCF-7 human breast cancer cells were used as the immunogen for this EpCAM antibody.
Clonality	Monoclonal
NCBI Official Name	epithelial cell adhesion molecule
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 9/4/2025

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