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Melanoma Antibody [NKI-beteb]

Melanoma Antibody [NKI-beteb]

Item no.	PRS-33-202-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Antibodies Flow Cytometry Immunohistochemistry Immunofluorescence Primary Antibodies By Organ Skin Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC
Clone	NKI-beteb
Specific against	Human (Homo sapiens), Horse (Equine)
Host	Mouse
Isotype	IgG2b Kappa
Conjugate/Tag	Unconjugated
NCBI	PMEL
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Melanocyte protein PMEL, ME20-M, ME20M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, M-alpha, 95 kDa melanocyte-specific secreted glycoprotein, P26, Secreted melanoma-associated ME20 antigen, ME20-S, ME20S, M-beta, PMEL, D12S53E, PMEL17, SILV
Available
Manufacturer - Applications	Flow Cytometry: 0.5-1 ug/million cells IF: 1-2 ug/ml IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1) Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer, Obesity, Signal Transduction
Background	By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Immunogen	Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
Clonality	Monoclonal
NCBI Official Name	premelanosome protein
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 9/4/2025

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