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Biotin Antibody [Hyb-8]

Biotin Antibody [Hyb-8]

Item no.	PRS-33-236-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Neuroscience Aging & Neurological Disorders Antibodies Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC
Clone	Hyb-8
Specific against	other
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Specificity	All species
Manufacturer - Type	Primary Antibodies
Manufacturer - Applications	WB, IHC, Flow, IF, CM
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Other
Background	This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.
By Type	Other
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml In situ Nucleic Acid Hybridization WB: 2-5 ug/ml Confocal Microscopy The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance. 1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Immunogen	Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
Clonality	Monoclonal
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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