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CD45RO Antibody [SPM125]

CD45RO Antibody [SPM125]

Item no.	PRS-33-305-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Neural Lineage Markers Antibodies By Cell Type Carcinoma Hematology Leukemia Flow Cytometry Immunohistochemistry Immunofluorescence Primary Antibodies Progenitor Markers
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC
Clone	SPM125
Specific against	Human (Homo sapiens), Marmoset
Host	Mouse
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
NCBI	PTPRC
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen,L-CA,T200,CD45,PTPRC,CD45
Shipping Condition	Cool pack
Available
Specificity	Human, Chimpanzee, Marmoset
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer, Immunology, Neuroscience, Signal Transduction
Background	Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
By Type	Cancer, Immunology, Neuroscience, Signal Transduction
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1 ug/million cells Immunofluorescence: 0.5-1 ug/ml Immunohistology (FFPE): 0.25-0.5 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance. 1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen	Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.
Clonality	Monoclonal
NCBI Official Name	protein tyrosine phosphatase, receptor type, C
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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