Comparison

Cytokeratin 7 Antibody [KRT7/903]

Item no. PRS-33-426-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IF, IHC-P
Clone KRT7/903
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
Conjugate/Tag Unconjugated
NCBI KRT7
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Keratin,type II cytoskeletal 7,Cytokeratin-7,CK-7,Keratin-7,K7,Sarcolectin,Type-II keratin Kb7,KRT7,SCL
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Signal Transduction
Background
Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
By Type
Signal Transduction
Modifications
None
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Manufacturer - Specificity
Does not react with Mouse, Rat, Ferret, rabbit
Immunogen
Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.
Clonality
Monoclonal
NCBI Official Name
keratin 7
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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