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MFG-E8 Antibody [SPM291]

MFG-E8 Antibody [SPM291]

Item no.	PRS-33-437-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Antibodies Cell Biology Cytoskeleton Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Intermediate filaments
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC-P
Clone	SPM291
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	Unconjugated
NCBI	MFGE8
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Lactadherin,Breast epithelial antigen BA46,HMFG,MFGM,Milk fat globule-EGF factor 8,MFG-E8,SED1,Lactadherin short form,Medin,MFGE8
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cell Cycle, Cell Cycle, Obesity,
Background	Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
By Type	Cell Cycle, Cell Cycle, Obesity,
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Western blot: 1-2 ug/ml Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the MFG-E8 antibody should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen	A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.
Clonality	Monoclonal
NCBI Official Name	milk fat globule-EGF factor 8 protein
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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