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GRP94 Antibody [HSP90B1/1192]

GRP94 Antibody [HSP90B1/1192]

Item no.	PRS-33-474-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Aging & Neurological Disorders Antibodies By Cell Type Carcinoma Sarcoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC-P
Clone	HSP90B1/1192
Specific against	Human (Homo sapiens)
Host	Rat
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
NCBI	HSP90B1
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Endoplasmin,94 kDa glucose-regulated protein,GRP-94,Heat shock protein 90 kDa beta member 1,Tumor rejection antigen 1,gp96 homolog,HSP90B1,GRP94,TRA1
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer, Obesity, Signal Transduction
Background	Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
By Type	Cancer, Obesity, Signal Transduction
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Western blot: 0.5-1 ug/ml Immunohistology (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Optimal dilution of the GRP94 antibody should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen	Recombinant full-length human protein was used as the immunogen for the GRP94 antibody.
Clonality	Monoclonal
NCBI Official Name	heat shock protein 90kDa beta (Grp94), member 1
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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