Calnexin Antibody [CANX/1541]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

None

0.2 mg/mL

WB: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10^6 cells
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT
Titering of the Calnexin antibody may be required for optimal performance.

A partial human recombinant protein was used as the immunogen for the Calnexin antibody.

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.