Integrin beta 3 Antibody [ITGB3/1713]

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

IHC (FFPE & Frozen): 1-2 ug/ml for 30 min at RT

Titration of the Integrin beta 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Reacts with human Integrin beta 3, also called CD61. It associates with the alphaV-chain (CD51) to form vitronectin receptor, or with the alphaIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

0.2 mg/mL

Human recombinant protein used as the immunogen for the Integrin beta 3 antibody.

integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.