Connexin 32 Antibody [GJB1/1753]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32, also called Gap junction protein beta 1. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.

None

0.2 mg/mL

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher