Thymidine Phosphorylase Antibody [P-GF.44C]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

None

0.2 mg/mL

IP: 1-2ug/500ug lysate
IHC (FFPE): 1-2 ug/ml for 30 min at RT
WB: 0.5-1 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidine Phosphorylase antibody to be titered up or down for optimal performance.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher