TSHR beta chain Antibody [SPM222]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

None

0.2 mg/mL

Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TSH Receptor antibody to be titered up or down for optimal performance.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher