Retinoblastoma / Rb Antibody [SPM353]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

None

0.2 mg/mL

WB: 0.5-1 ug/ml
IP: 1-2ug/500ug protein lysate
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Titering of the Retinoblastoma antibody may be required for optimal performance.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher