CD81 Antibody [1.3.3.22]

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

WB: 0.5-1 ug/ml

Functional Studies: order BSA free formulation

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

Optimal dilution of the CD81 antibody should be determined by the researcher.

Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

This mAb recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

0.2 mg/mL

A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the CD81 antibody.

CD81 molecule

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.