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Recombinant Basic Cytokeratin Antibody [KRTH/1576R]

Recombinant Basic Cytokeratin Antibody [KRTH/1576R]

Item no.	PRS-33-833-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Neuroinflammation Antibodies By Cell Type Hematology Leukemia Cell Biology Cytoskeleton Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Intermediate filaments By Organ Heart & Blood Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC-P
Clone	KRTH/1576R
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
NCBI	KRT76
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	KRT2B,KRT2P,Keratin,type II cytoskeletal 2 oral,Cytokeratin-2P,CK-2P,HUMCYT2A
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Manufacturer - Isotype	IgG, k
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Signal Transduction
Background	This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
By Type	Signal Transduction
Badges	Recombinant
Modifications	None
Purification	Protein A affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Western blot: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen	Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.
Clonality	Recombinant Monoclonal
NCBI Official Name	keratin 76
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 11/6/2025

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