Comparison

ER Antibody [NR3Ga-3]

Item no. PRS-33-910-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IHC
Clone NR3Ga-3
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Conjugate/Tag Unconjugated
NCBI ESR1
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias ESR1 Antibody: ER,ESR,Era,ESRA,ESTRR,NR3A1,Estrogen receptor,ER-alpha,ER
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Signal Transduction
Background
Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.
By Type
Signal Transduction
Modifications
None
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Western blot: 1-2 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IHC (Formalin-paraffin): 1-2 ug/ml (1)
Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.

1. FFPE testing requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min and cooling at RT for 20 minutes.

Immunogen
Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
Clonality
Monoclonal
NCBI Official Name
estrogen receptor 1
NCBI Organism
homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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