HER2 Antibody [2KRI7]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

HER2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. HER2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary. [RefSeq]

None

0.2 mg/mL

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at Room Temp (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER2 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher