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SM22 alpha Antibody [SMP22a]

SM22 alpha Antibody [SMP22a]

Item no.	PRS-33-977-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Neural Lineage Markers Antibodies By Cell Type Carcinoma Sarcoma Cell Biology Cytoskeleton Immunohistochemistry Immunofluorescence Primary Antibodies Intermediate filaments By Organ Breast Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	IF, IHC-P
Clone	SMP22a
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	Unconjugated
NCBI	TAGLN
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Transgelin,22 kDa actin-binding protein,Protein WS3-10,Smooth muscle protein 22-alpha,SM22-alpha,TAGLN,SM22,WS3-10
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Signal Transduction
Background	SM22/Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart.
By Type	Signal Transduction
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the SM22 alpha antibody may be required for optimal performance. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. 3. Cross-reaction with SM22 beta protein has not been determined.
Immunogen	Recombinant full-length human protein was used as the immunogen for the SM22 alpha antibody. (3)
Clonality	Monoclonal
NCBI Official Name	transgelin
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 11/6/2025

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