VCAM-1 Antibody [CDLA106]

Item no.	PRS-33-994-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Neuroscience Neuroinflammation Antibodies Cell Biology Cell Movement Cell Adhesion Flow Cytometry Immunohistochemistry Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC-P
Clone	CDLA106
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	Unconjugated
NCBI	VCAM1
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Vascular cell adhesion protein 1,V-CAM 1,VCAM-1,INCAM-100,CD106,VCAM1,L1CAM
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer, Neuroscience, Signal Transduction
Background	Vascular cell adhesion molecule-1 (VCAM-1), also called L1CAM and CD106, is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt]
By Type	Cancer, Neuroscience, Signal Transduction
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the VCAM-1 antibody may be required for optimal performance. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen	Recombinant human protein was used as the immunogen for the VCAM-1 antibody.
Clonality	Monoclonal
NCBI Official Name	vascular cell adhesion molecule 1
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 11/6/2025

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