Comparison

Recombinant CD99 Antibody [MIC2/1495R]

Item no. PRS-34-074-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications FC, IF, IHC-P
Clone MIC2/1495R
Specific against Human (Homo sapiens)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
NCBI CD99
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias CD99,12E7,CD99 antigen,MIC2X,MIC2,Surface antigen MIC2,MIC2Y,MSK5X,Protein MIC2,T-cell surface glycoprotein E2,CD99 molecule,E2 antigen,HBA71
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Manufacturer - Isotype
IgG, kappa
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Signal Transduction
Background
CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]
By Type
Signal Transduction
Badges
Recombinant
Modifications
None
Purification
Protein A affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Flow Cytometry: 2-4 ug/million cells in 0.1ml
Immunofluorescence: 2-4 ug/ml
Immunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Titering of the recombinant CD99 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen
Human protein was used as the immunogen for the recombinant CD99 antibody.
Clonality
Recombinant Monoclonal
NCBI Official Name
CD99 molecule
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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