Comparison

Recombinant CD15 Antibody [FUT4/1478R]

Item no. PRS-34-075-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications FC, IF, IHC-P
Clone FUT4/1478R
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
NCBI FUT4
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Alpha-(1,3)-fucosyltransferase 4,241-,ELAM-1 ligand fucosyltransferase,Fucosyltransferase 4,Fucosyltransferase IV,Fuc-TIV,FucT-IV,Galactoside 3-L-fucosyltransferase,FUT4,ELFT,FCT3A
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Manufacturer - Isotype
IgG, k
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Cancer, Cell Cycle, Immunology, Signal Transduction
Background
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
By Type
Cancer, Cell Cycle, Immunology, Signal Transduction
Badges
Recombinant
Modifications
None
Purification
Protein A affinity
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Flow Cytometry: 2-4 ug/million cells in 0.1ml
Immunofluorescence: 2-4 ug/ml
Immunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Optimal dilution of the recombinant CD15 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen
Purified human neutrophils were used as the immunogen for the recombinant CD15 antibody.
Clonality
Recombinant Monoclonal
NCBI Official Name
fucosyltransferase 4 (alpha (1, 3) fucosyltransferase, myeloid-specific)
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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