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PCNA Antibody [SPM350]

PCNA Antibody [SPM350]

Item no.	PRS-34-150-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Neural Adhesion Molecules Antibodies By Cell Type Blastoma Carcinoma Hematology Myeloma Sarcoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies By Organ Lung Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC
Clone	SPM350
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Monkey (Cynomolgus, Simian), Chicken (Gallus gallus domesticus), Pig (Porcine, Sus scrofa domesticus), Zebrafish (Danio rerio), Drosophila, Yeast (Saccharomyces cerevisiae)
Host	Mouse
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
NCBI	PCNA
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Proliferating cell nuclear antigen,PCNA,Cyclin,PCNA
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer, Cell Cycle
Background	Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
By Type	Cancer, Cell Cycle
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Western blot: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen	Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.
Clonality	Monoclonal
NCBI Official Name	proliferating cell nuclear antigen
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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