Comparison

Bcl-X Antibody [SPM165]

Item no. PRS-34-161-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IF, IHC
Clone SPM165
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Pig (Porcine, Sus scrofa domesticus)
Host Mouse
Isotype IgG2a
Conjugate/Tag Unconjugated
NCBI BCL2L1
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Bcl-xL Antibody: BCLX,BCL2L,BCLXL,BCLXS,Bcl-X,bcl-xL,bcl-xS,PPP1R52,BCL-XL/S,BCLX,Bcl-2-like protein 1,Apoptosis regulator Bcl-X,Bcl2-L-1
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Apoptosis
Background
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.
By Type
Apoptosis
Modifications
None
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The optimal dilution of the anti-Bcl-X antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen
Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this anti-Bcl-X antibody.
Clonality
Monoclonal
NCBI Official Name
BCL2-like 1
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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