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p53 Antibody [SPM589]

p53 Antibody [SPM589]

Item no.	PRS-34-173-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Food Safety Neuroscience Aging & Neurological Disorders Antibodies By Cell Type Hematology Leukemia Lymphoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Contaminants Primary Antibodies Metals By Organ Skin Cancer Renal Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC
Clone	SPM589
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2a
Conjugate/Tag	Unconjugated
NCBI	TP53
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
Available
Manufacturer - Applications	Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Western blot: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer, Cell Cycle, Signal Transduction,
Background	p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Immunogen	Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25).
Clonality	Monoclonal
NCBI Official Name	tumor protein p53
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 9/4/2025

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