Biotin Antibody [SPM375]

Item no.	PRS-34-197-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Antibodies By Cell Type Hematology Leukemia Lymphoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC, FA
Clone	SPM375
Specific against	other
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Specificity	All species
Manufacturer - Type	Primary Antibodies
Manufacturer - Applications	Flow, IF, WB, IHC, Functional Assays, CM
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Other
Background	It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
By Type	Other
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Confocal Microscopy Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml In situ Nucleic Acid Hybridization Western: blot 1-3 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher. 1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Immunogen	Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
Clonality	Monoclonal
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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