GITRL Antibody

ELISA: (direct and indirect: 1:5, 000-1:10, 000). Western Blot: (1:2, 000-1:5, 000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Stable for at least 6 months after receipt when stored at -20°C.

GITRL is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e. g. testis, . ). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T-cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.

1 mg/ml

Recombinant human GITRL.

tumor necrosis factor (ligand) superfamily, member 18

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.