sTNF-receptor II Antibody

Primary Antibodies, Functional Antibodies, Antibody Pairs

Room Temp

Immunology, Growth Factor, Apoptosis, Antibody Pairs, Functional Assays

Immunology, Growth Factors, Apoptosis

21264534

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human sTNF Receptor-II (0.5 μ g/mL) a concentration of 15.0 - 20.0 μ g/mL of this antibody is required.
ELISA:
Indirect:
To detect hsTNF-receptor II by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.
Sandwich
To detect hsTNF-receptor II by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human sTNF-receptor II as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II.
Western Blot:
To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Homo sapiens

Centrifuge vial prior to opening.