Comparison

WNT-3a Antibody

Item no. PRS-38-177-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Lyophilized
Applications WB, ELISA
Specific against Human (Homo sapiens)
Host Rabbit
Conjugate/Tag Unconjugated
NCBI WNT3A
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Protein Wnt-3a
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary Antibodies, Antibody Pairs
Shipping Temperature
Room Temp
Storage Conditions
WNT-3a antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.
Manufacturer - Research Area
Stem Cell, Neuroscience, Antibody Pairs
Background
Wnt3a is a secreted ligand for members of the Frizzled family of receptors. It is one of the closely-related Wnt family and has multiple roles in development and cell fate.
By Type
Stem Cell, Neuroscience
Modifications
None
Protein Gi #
20532424
Concentration
batch dependent
Application Note
ELISA:
Indirect:
To detect hWNT-3a by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
Sandwich
To detect hWNT-3a by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human WNT-3a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
Western Blot:
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography employing immobilized hWNT-3a matrix.
Clonality
Polyclonal
NCBI Official Name
wingless-type MMTV integration site family, member 3A
NCBI Organism
Homo sapiens
Disclaimer
This product is for research use only.
User Note
Centrifuge vial prior to opening.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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