Comparison

SF-20 Antibody

Item no. PRS-38-189-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Lyophilized
Applications WB, ELISA
Specific against Mouse (Murine, Mus musculus)
Host Rabbit
Conjugate/Tag Unconjugated
NCBI D17Wsu104e
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Il25,Ly6elg,Il25,UPF0556 protein C19orf10 homolog,Interleukin-25,IL-25
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary Antibodies, Antibody Pairs
Shipping Temperature
Room Temp
Storage Conditions
SF-20 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.
Manufacturer - Research Area
Antibody Pairs
Background
SF20, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown
By Type
Other
Modifications
None
Protein Gi #
61221740
Concentration
batch dependent
Application Note
ELISA:
Indirect:
To detect mSF-20 by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.
Sandwich
To detect mSF-20 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine SF-20 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20. Western Blot To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSF-20. Murine SF-20 specific antibody was purified by affinity chromatography employing immobilized mSF-20 matrix.
Clonality
Polyclonal
NCBI Official Name
DNA segment, Chr 17, Wayne State University 104, expressed
NCBI Organism
Mus musculus
Disclaimer
This product is for research use only.
User Note
Centrifuge vial prior to opening.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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