Comparison

IL-2 Antibody (biotin)

Item no. PRS-38-205-0.05mg
Manufacturer ProSci
Amount 0.05 mg
Category
Type Antibody Primary
Format Lyophilized
Applications WB, ELISA
Specific against Rat (Rattus norvegicus)
Host Rabbit
Conjugate/Tag Biotin
NCBI Il2
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Il-2,Interleukin-2,T-cell growth factor,IL-2
Shipping Condition Room temperature
Available
Manufacturer - Type
Primary Antibodies, Antibody Pairs
Shipping Temperature
Room Temp
Storage Conditions
IL-2 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.
Manufacturer - Research Area
Immunology, Chemokines & Cytokines, Antibody Pairs
Background
Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
By Type
Immunology, Chemokines & Cytokines
Modifications
None
Protein Gi #
124328
Concentration
batch dependent
Application Note
ELISA:
Sandwich:
To detect Rat IL-2 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Rat IL-2 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Western Blot:
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-2. Rat IL-2 specific antibody was purified by affinity chromatography and then biotinylated.
Clonality
Polyclonal
NCBI Official Name
interleukin 2
NCBI Organism
Rattus norvegicustus norvegicus
Disclaimer
This product is for research use only.
User Note
Centrifuge vial prior to opening.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.05 mg
Available: In stock
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