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EGF Antibody

EGF Antibody

Item no.	PRS-38-210-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Neuroscience Neural Signaling Antibodies Western Blot ELISA Primary Antibodies By Organ Bladder Cancer
Type	Antibody Primary
Format	Lyophilized
Applications	WB, ELISA, NT
Specific against	Rat (Rattus norvegicus)
Host	Rabbit
Conjugate/Tag	Unconjugated
NCBI	Egf
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Pro-epidermal growth factorEGF
Shipping Condition	Room temperature
Available
Manufacturer - Type	Primary Antibodies, Functional Antibodies, Antibody Pairs
Manufacturer - Applications	ELISA, WB, Neutralization Assay
Shipping Temperature	Room Temp
Storage Conditions	AntiEGF antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.
Manufacturer - Research Area	Growth Factor, Antibody Pairs, Functional Assays
Background	Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor is also a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways
By Type	Growth Factors
Modifications	None
Protein Gi #	1352360
Concentration	batch dependent
Application Note	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat EGF (0.1 ng/mL), a concentration of < 0.1 μ g/mL of this antibody is required. ELISA: Indirect: To detect Rat EGF by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF. Sandwich To detect Rat EGF by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat EGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF. Western Blot: To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunogen	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Rat EGF specific antibody was purified by affinity chromatography employing immobilized Rat EGF matrix.
Clonality	Polyclonal
NCBI Official Name	epidermal growth factor
NCBI Organism	Rattus norvegicustus norvegicus
Disclaimer	This product is for research use only.
User Note	Centrifuge vial prior to opening.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 12/4/2025

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