IL-2 Antibody [5B9-2-1]

Primary Antibodies, Functional Antibodies

Room Temp

Infectious Disease, Neuroscience, Cancer, Stem Cell, Chemokines & Cytokines, Functional Assays

None

This IgG2Ak antibody was purified from cell culture by Protein A affinity chromatography.

ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μ L/well), a concentration of 4.0-8.0 μ g/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μ g/mL.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μ g/mL of this antibody is required.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.